Review





Similar Products

goat antihuman igg fitc  (Native Antigen Inc)
94
Native Antigen Inc goat antihuman igg fitc
Goat Antihuman Igg Fitc, supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat antihuman igg fitc/product/Native Antigen Inc
Average 94 stars, based on 1 article reviews
goat antihuman igg fitc - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
pbs t hrp conjugated goat antihuman secondary antibody  (Valiant Co Ltd)
96
Valiant Co Ltd pbs t hrp conjugated goat antihuman secondary antibody
Pbs T Hrp Conjugated Goat Antihuman Secondary Antibody, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs t hrp conjugated goat antihuman secondary antibody/product/Valiant Co Ltd
Average 96 stars, based on 1 article reviews
pbs t hrp conjugated goat antihuman secondary antibody - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
fluorescein isothiocyanate conjugated antihuman complement c3  (Valiant Co Ltd)
94
Valiant Co Ltd fluorescein isothiocyanate conjugated antihuman complement c3
Induction of neutrophil ROS by S . Typhimurium depends on <t>complement.</t> (A) Production of ROS over time by S . Typhimurium-infected HL-60 cells incubated with normal human serum or with the complement inhibitor Futhan. Data shown are combined from seven independent experiments. ( B ) Average chemiluminescence from seven independent experiments, 40 min after addition of bacteria at a multiplicity of infection of 10. ( C ) 4/74 and its laboratory derivative SL1344 elicit identical levels of ROS from neutrophilic HL-60 cells. Data shown are from four independent experiments. ( D ) Quantification of complement factor <t>C3</t> binding on the surface of S . Typhimurium after opsonization with 1%, 5%, or 10% of human serum. Shown is a representative experiment, with C3-positive events designated by a horizontal line. Percentage of C3-positive bacteria for each strain is given in the upper right of each plot. ( E ) Average relative C3 binding on the surface of S . Typhimurium after opsonization with 10% serum was measured by flow cytometry. Data shown are from five independent experiments. Each dot represents the mean of technical replicates from a single experiment. Mock, mock infection; NHS, normal human serum; ns, not significant; RLU, relative luminescence unit. Differences between groups were determined using a Mann-Whitney test. **, P < 0.01.
Fluorescein Isothiocyanate Conjugated Antihuman Complement C3, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluorescein isothiocyanate conjugated antihuman complement c3/product/Valiant Co Ltd
Average 94 stars, based on 1 article reviews
fluorescein isothiocyanate conjugated antihuman complement c3 - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
goat-antihuman horseradish peroxidase-conjugated secondary antibody  (Jackson Immuno)
90
Jackson Immuno goat-antihuman horseradish peroxidase-conjugated secondary antibody
Induction of neutrophil ROS by S . Typhimurium depends on <t>complement.</t> (A) Production of ROS over time by S . Typhimurium-infected HL-60 cells incubated with normal human serum or with the complement inhibitor Futhan. Data shown are combined from seven independent experiments. ( B ) Average chemiluminescence from seven independent experiments, 40 min after addition of bacteria at a multiplicity of infection of 10. ( C ) 4/74 and its laboratory derivative SL1344 elicit identical levels of ROS from neutrophilic HL-60 cells. Data shown are from four independent experiments. ( D ) Quantification of complement factor <t>C3</t> binding on the surface of S . Typhimurium after opsonization with 1%, 5%, or 10% of human serum. Shown is a representative experiment, with C3-positive events designated by a horizontal line. Percentage of C3-positive bacteria for each strain is given in the upper right of each plot. ( E ) Average relative C3 binding on the surface of S . Typhimurium after opsonization with 10% serum was measured by flow cytometry. Data shown are from five independent experiments. Each dot represents the mean of technical replicates from a single experiment. Mock, mock infection; NHS, normal human serum; ns, not significant; RLU, relative luminescence unit. Differences between groups were determined using a Mann-Whitney test. **, P < 0.01.
Goat Antihuman Horseradish Peroxidase Conjugated Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat-antihuman horseradish peroxidase-conjugated secondary antibody/product/Jackson Immuno
Average 90 stars, based on 1 article reviews
goat-antihuman horseradish peroxidase-conjugated secondary antibody - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
r-phycoerythrin-conjugated goat antihuman igg secondary antibody 109–115-098  (Jackson Immuno)
90
Jackson Immuno r-phycoerythrin-conjugated goat antihuman igg secondary antibody 109–115-098
Induction of neutrophil ROS by S . Typhimurium depends on <t>complement.</t> (A) Production of ROS over time by S . Typhimurium-infected HL-60 cells incubated with normal human serum or with the complement inhibitor Futhan. Data shown are combined from seven independent experiments. ( B ) Average chemiluminescence from seven independent experiments, 40 min after addition of bacteria at a multiplicity of infection of 10. ( C ) 4/74 and its laboratory derivative SL1344 elicit identical levels of ROS from neutrophilic HL-60 cells. Data shown are from four independent experiments. ( D ) Quantification of complement factor <t>C3</t> binding on the surface of S . Typhimurium after opsonization with 1%, 5%, or 10% of human serum. Shown is a representative experiment, with C3-positive events designated by a horizontal line. Percentage of C3-positive bacteria for each strain is given in the upper right of each plot. ( E ) Average relative C3 binding on the surface of S . Typhimurium after opsonization with 10% serum was measured by flow cytometry. Data shown are from five independent experiments. Each dot represents the mean of technical replicates from a single experiment. Mock, mock infection; NHS, normal human serum; ns, not significant; RLU, relative luminescence unit. Differences between groups were determined using a Mann-Whitney test. **, P < 0.01.
R Phycoerythrin Conjugated Goat Antihuman Igg Secondary Antibody 109–115 098, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/r-phycoerythrin-conjugated goat antihuman igg secondary antibody 109–115-098/product/Jackson Immuno
Average 90 stars, based on 1 article reviews
r-phycoerythrin-conjugated goat antihuman igg secondary antibody 109–115-098 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
goat antihuman igg  (Jackson Immuno)
96
Jackson Immuno goat antihuman igg
Induction of neutrophil ROS by S . Typhimurium depends on <t>complement.</t> (A) Production of ROS over time by S . Typhimurium-infected HL-60 cells incubated with normal human serum or with the complement inhibitor Futhan. Data shown are combined from seven independent experiments. ( B ) Average chemiluminescence from seven independent experiments, 40 min after addition of bacteria at a multiplicity of infection of 10. ( C ) 4/74 and its laboratory derivative SL1344 elicit identical levels of ROS from neutrophilic HL-60 cells. Data shown are from four independent experiments. ( D ) Quantification of complement factor <t>C3</t> binding on the surface of S . Typhimurium after opsonization with 1%, 5%, or 10% of human serum. Shown is a representative experiment, with C3-positive events designated by a horizontal line. Percentage of C3-positive bacteria for each strain is given in the upper right of each plot. ( E ) Average relative C3 binding on the surface of S . Typhimurium after opsonization with 10% serum was measured by flow cytometry. Data shown are from five independent experiments. Each dot represents the mean of technical replicates from a single experiment. Mock, mock infection; NHS, normal human serum; ns, not significant; RLU, relative luminescence unit. Differences between groups were determined using a Mann-Whitney test. **, P < 0.01.
Goat Antihuman Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat antihuman igg/product/Jackson Immuno
Average 96 stars, based on 1 article reviews
goat antihuman igg - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
goat antihuman igg hrp  (SouthernBiotech)
97
SouthernBiotech goat antihuman igg hrp
Induction of neutrophil ROS by S . Typhimurium depends on <t>complement.</t> (A) Production of ROS over time by S . Typhimurium-infected HL-60 cells incubated with normal human serum or with the complement inhibitor Futhan. Data shown are combined from seven independent experiments. ( B ) Average chemiluminescence from seven independent experiments, 40 min after addition of bacteria at a multiplicity of infection of 10. ( C ) 4/74 and its laboratory derivative SL1344 elicit identical levels of ROS from neutrophilic HL-60 cells. Data shown are from four independent experiments. ( D ) Quantification of complement factor <t>C3</t> binding on the surface of S . Typhimurium after opsonization with 1%, 5%, or 10% of human serum. Shown is a representative experiment, with C3-positive events designated by a horizontal line. Percentage of C3-positive bacteria for each strain is given in the upper right of each plot. ( E ) Average relative C3 binding on the surface of S . Typhimurium after opsonization with 10% serum was measured by flow cytometry. Data shown are from five independent experiments. Each dot represents the mean of technical replicates from a single experiment. Mock, mock infection; NHS, normal human serum; ns, not significant; RLU, relative luminescence unit. Differences between groups were determined using a Mann-Whitney test. **, P < 0.01.
Goat Antihuman Igg Hrp, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat antihuman igg hrp/product/SouthernBiotech
Average 97 stars, based on 1 article reviews
goat antihuman igg hrp - by Bioz Stars, 2026-03
97/100 stars
  Buy from Supplier
alexa fluor 647 affinipure goat antihuman igg probe  (Jackson Immuno)
94
Jackson Immuno alexa fluor 647 affinipure goat antihuman igg probe
Induction of neutrophil ROS by S . Typhimurium depends on <t>complement.</t> (A) Production of ROS over time by S . Typhimurium-infected HL-60 cells incubated with normal human serum or with the complement inhibitor Futhan. Data shown are combined from seven independent experiments. ( B ) Average chemiluminescence from seven independent experiments, 40 min after addition of bacteria at a multiplicity of infection of 10. ( C ) 4/74 and its laboratory derivative SL1344 elicit identical levels of ROS from neutrophilic HL-60 cells. Data shown are from four independent experiments. ( D ) Quantification of complement factor <t>C3</t> binding on the surface of S . Typhimurium after opsonization with 1%, 5%, or 10% of human serum. Shown is a representative experiment, with C3-positive events designated by a horizontal line. Percentage of C3-positive bacteria for each strain is given in the upper right of each plot. ( E ) Average relative C3 binding on the surface of S . Typhimurium after opsonization with 10% serum was measured by flow cytometry. Data shown are from five independent experiments. Each dot represents the mean of technical replicates from a single experiment. Mock, mock infection; NHS, normal human serum; ns, not significant; RLU, relative luminescence unit. Differences between groups were determined using a Mann-Whitney test. **, P < 0.01.
Alexa Fluor 647 Affinipure Goat Antihuman Igg Probe, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alexa fluor 647 affinipure goat antihuman igg probe/product/Jackson Immuno
Average 94 stars, based on 1 article reviews
alexa fluor 647 affinipure goat antihuman igg probe - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
polyclonal goat antihuman alexa fluor plus 488-conjugated igg  (Thermo Fisher)
90
Thermo Fisher polyclonal goat antihuman alexa fluor plus 488-conjugated igg
Induction of neutrophil ROS by S . Typhimurium depends on <t>complement.</t> (A) Production of ROS over time by S . Typhimurium-infected HL-60 cells incubated with normal human serum or with the complement inhibitor Futhan. Data shown are combined from seven independent experiments. ( B ) Average chemiluminescence from seven independent experiments, 40 min after addition of bacteria at a multiplicity of infection of 10. ( C ) 4/74 and its laboratory derivative SL1344 elicit identical levels of ROS from neutrophilic HL-60 cells. Data shown are from four independent experiments. ( D ) Quantification of complement factor <t>C3</t> binding on the surface of S . Typhimurium after opsonization with 1%, 5%, or 10% of human serum. Shown is a representative experiment, with C3-positive events designated by a horizontal line. Percentage of C3-positive bacteria for each strain is given in the upper right of each plot. ( E ) Average relative C3 binding on the surface of S . Typhimurium after opsonization with 10% serum was measured by flow cytometry. Data shown are from five independent experiments. Each dot represents the mean of technical replicates from a single experiment. Mock, mock infection; NHS, normal human serum; ns, not significant; RLU, relative luminescence unit. Differences between groups were determined using a Mann-Whitney test. **, P < 0.01.
Polyclonal Goat Antihuman Alexa Fluor Plus 488 Conjugated Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyclonal goat antihuman alexa fluor plus 488-conjugated igg/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
polyclonal goat antihuman alexa fluor plus 488-conjugated igg - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Induction of neutrophil ROS by S . Typhimurium depends on complement. (A) Production of ROS over time by S . Typhimurium-infected HL-60 cells incubated with normal human serum or with the complement inhibitor Futhan. Data shown are combined from seven independent experiments. ( B ) Average chemiluminescence from seven independent experiments, 40 min after addition of bacteria at a multiplicity of infection of 10. ( C ) 4/74 and its laboratory derivative SL1344 elicit identical levels of ROS from neutrophilic HL-60 cells. Data shown are from four independent experiments. ( D ) Quantification of complement factor C3 binding on the surface of S . Typhimurium after opsonization with 1%, 5%, or 10% of human serum. Shown is a representative experiment, with C3-positive events designated by a horizontal line. Percentage of C3-positive bacteria for each strain is given in the upper right of each plot. ( E ) Average relative C3 binding on the surface of S . Typhimurium after opsonization with 10% serum was measured by flow cytometry. Data shown are from five independent experiments. Each dot represents the mean of technical replicates from a single experiment. Mock, mock infection; NHS, normal human serum; ns, not significant; RLU, relative luminescence unit. Differences between groups were determined using a Mann-Whitney test. **, P < 0.01.

Journal: mBio

Article Title: African Salmonella enterica serovar Typhimurium ST313 isolates prevent reactive oxygen species production by human neutrophils via elevated PgtE expression

doi: 10.1128/mbio.01609-25

Figure Lengend Snippet: Induction of neutrophil ROS by S . Typhimurium depends on complement. (A) Production of ROS over time by S . Typhimurium-infected HL-60 cells incubated with normal human serum or with the complement inhibitor Futhan. Data shown are combined from seven independent experiments. ( B ) Average chemiluminescence from seven independent experiments, 40 min after addition of bacteria at a multiplicity of infection of 10. ( C ) 4/74 and its laboratory derivative SL1344 elicit identical levels of ROS from neutrophilic HL-60 cells. Data shown are from four independent experiments. ( D ) Quantification of complement factor C3 binding on the surface of S . Typhimurium after opsonization with 1%, 5%, or 10% of human serum. Shown is a representative experiment, with C3-positive events designated by a horizontal line. Percentage of C3-positive bacteria for each strain is given in the upper right of each plot. ( E ) Average relative C3 binding on the surface of S . Typhimurium after opsonization with 10% serum was measured by flow cytometry. Data shown are from five independent experiments. Each dot represents the mean of technical replicates from a single experiment. Mock, mock infection; NHS, normal human serum; ns, not significant; RLU, relative luminescence unit. Differences between groups were determined using a Mann-Whitney test. **, P < 0.01.

Article Snippet: Deposited C3 on the bacteria was stained by incubation with fluorescein isothiocyanate-conjugated antihuman complement C3 (MP Biomedicals, SKU: 0855167) for 1 h. Stained samples were analyzed by a flow cytometer (Cytek, Aurora), and cell populations were quantified using FlowJo software (Becton Dickinson and Company).

Techniques: Infection, Incubation, Bacteria, Binding Assay, Flow Cytometry, MANN-WHITNEY